Imaging cytometry is a powerful technology that combines classical flow cytometry with fluorescence microscopy into a single platform. Such integration is possible thanks to the use of charge-coupled device (CCD) camera technology and a patented time-delay integration system, which provides exceptional signal detection sensitivity unmatched by other instruments.  

Thus, ImageStream System enables high-throughput analysis of cells and nanoparticles in suspension, coupled with the collection of images of each analyzed object in several detection channels. That allows to perform rapid, multi-parameter cytometric analysis, with the simultaneous verification of objects through their actual images. Moreover, it also enables the quantitative and qualitative analysis of a range of morphometric parameters of individual objects. This technology is particularly important in the context of research on rare cell populations, as well as EV analysis, which require exceptional sensitivity and resolution. 

Our ImageStreamX Mk II is an integrated system equipped with five lasers and two synchronized CCD cameras, enabling the imaging of individual objects across 12 detection channels. The camera also features a MultiMag system, enabling operation with 20x, 40x, and 60x objectives, providing extensive capabilities for obtaining high resolution across various types of analyzed samples. Of particular note, this system enables both detection and imaging of individual EVs, a feat unattainable with conventional cytometers. Additionally, the camera features an Extended Depth of Field (EDF) option to achieve the best possible focal plane. 

It is a unique, high-sensitivity and high-resolution flow cytometer dedicated for the analysis of small particles, with special regard to EVs. 

Our system is equipped with two lasers (488nm and 638nm), enabling particle measurement across six fluorescence detection channels, enabling multi-parameter phenotyping of nanoparticles present in the analyzed samples. Furthermore, it features a unique scattered light detector system, providing sensitivity unmatched by traditional flow cytometers, enabling the detection of particles as small as 80 nm in diameter. This makes it an optimal platform for EV analysis. 

An additional advantage of this cytometer is the micro-syringe sampling system, which allows for the direct counting of the absolute number of analyzed particles without the need for reference beads. 

It is FACS-based system that enables both high-throughput multi-parameter analysis of cells and their sorting. This comprehensive instrument features three lasers, enabling simultaneous analysis across 12 detection channels and efficient sorting of cells into up to 4 highly purified fractions. The sorter also features nozzles of varying diameters, allowing for the selection of optimal parameters for the isolation of various types of biological objects.  

Our instrument is also equipped with a module enabling single-cell sorting with simultaneous thermostatic control. The sorter also features a safety system that removes aerosols from the sorting chamber. 

Our laboratory is equipped in the NanoSight NS300 system that uses NTA technology relying on the analysis of the Brownian motion trajectories of nanoparticles in solution, tracked over time thanks to the sCMOS camera-based detection of signal coming from the laser beam that is scattered on the individual objects. The principle of NTA method bases on the Stokes-Einstein equation – the larger the particle, the slower it moves. 

NanoSight system operates by directly detecting and visualizing individual nanoparticles in a polydisperse suspension, which allows rapid, automated measurement of their size distribution and concentration. Additionally, using lasers and fluorescence detectors, the instrument also enables quantitative analyses of nanoparticles with specific expression of a selectable marker. This instrument is widely used not only in EV-based studies, but also in protein aggregation assays and the analysis of other organic and inorganic nanoparticles. 

Our NanoSight NS300 system is equipped with three interchangeable  laser modules and sCMOS camera, ensuring high image quality and speed. It also has a temperature control module to ensure stable measurement conditions, as well as a syringe pump, enabling not only a precise sample dosing, but also measurements in both stationary and controlled flow modes.